Oxidoreductase-metalloenzymes: Catalysis in green chemistry domain

Title : Oxidoreductase-metalloenzymes: Catalysis in green chemistry domain

Abstract:

Metalloenzymes namely lignin peroxidases, manganese peroxidases, versatile peroxidases, peroxidases and catalases are oxidoreductase group of enzymes that catalyses H2 O2 dependent oxidation reactions. Discussion will deal on kinetics of enzyme catalyzed reaction with respect to aims of enzyme kinetic studies, steady state and transient state kinetics, enzyme activity measurements. These specific enzymes will be taken into account with respect to their isolation, different techniques used in purification of enzyme from crude extract, determination of enzyme characteristic parameters like Michelis-menten constant (Km), turnover number (Kcat), Kcat/km, Vmax, pH optima and temperature optima and nature of inhibition studies will be discussed. Mechanism of action, Immobilization studies on beads isolated from crustacean species and application of these enzymes in delignification of lignin containing cellulose materials, the degradation of recalcitrant organic pollutants such as phenol containing compounds, pesticides, azodyes, and polyaromatic hydrocarbons (PAHs), development of enzyme-based biosensors for monitoring of H2 O2 and other hazardous wastes, and remediation of wastewater achieved by enzyme based immobilized micromotors, decolorization of pollutant dyes, soil treatment and conversion of high molecular weight coal fractions to low molecular weight coal fractions will be discussed. Consequently these enzymes provide very efficient tools for the removal and control of high H2 O2 levels. Provide an understanding of role of an enzyme in metabolic pathways. Studies will be helpful to sort out complex kinetic mechanism, regulatory strategies of enzyme, identify new enzyme inhibitors and measure their potency for development of drugs.

Audience Take Away:
• Enzyme technology will provide efficient tools for removal of pollutants that persist in environment.
• Studies will be helpful to sort out complex kinetic mechanism and regulatory strategies of enzyme.
• Identify new enzyme inhibitors and measure their potency for development of drugs.

Biography:

Dr. Meera Yadav completed her Master’s and Ph.D in Chemistry from DDU Gorakhpur University, Gorakhpur, U.P. India. She was awarded CSIR-SRF, twice as DST- Woman scientist A and UGC- Dr. D. S Kothari Postdoctoral Fellow at Department of Chemistry, Gorakhpur University, Gorakhpur. Her research area includes, purification, characterization and applications of Metalloenzymes namely Lignin peroxidase, Mn-peroxidase, Laccase and Chloroperoxidase. Use of enzymes in synthetic inorganic and organic chemistry. Currently she is working as an Assistant professor in the Dept. of Chemistry, North Eastern Regional Institute of Science and Technology (NERIST), Nirjuli, Itanagar, A.P., India. She has participated in more than 30 national and international seminars and conferences. She has guided two Ph.D and five students are being involved in research activities and has published more than 30 research papers in reputed national and international peer reviewed journals, 3 books, 6 chapters in reputed standard books. She is life member of American Chemical Society, Indian Council of Chemists and Indian Society of Chemists and Biologists.